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KMID : 0377520030280020135
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Chung-Ang Journal of Medicine
2003 Volume.28 No. 2 p.135 ~ p.142
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Expression of Factor Ⅷ-Related Antigen and Vascular Endothelial Growth Factor during Diethylnitrosamine-Induced Hepatocarcinogenesis in Rat liver
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Kim Hyung-Jun
Ki Seung-Suk
Park Yong-Jin
Kim Nam-Don
Kyun Jong-Man
Park Eon-Sub
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Abstract
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It is widely believed that chemical carcinogenesis is a multistage process, so-called initiation, promotion and propagation. Diethylnitrosamine (DEN) has been proved to have carcinogenic potential either at initiation or at promotion stage, resulting hepatocellular carcinomas in rat liver. In addition, neoplastic and metastatic propagation of transformed cells exclusively need a plenty of vascular supply for their metabolic requirement. To elucidate the tumor angiogenesis during the chemically induced carcinogenesis in rat liver, analysis of histopathology and the expression of vascular endothelial growth factor (VEGF) and Factor VⅢ related antigen were examined by immunohistochemical study using avidine-biotin complex. The hyperplastic nodules appeared at 3 weeks, the hepatocellular carcinomas were 15 weeks, and the pulmonary metastatic foci were developed at 18 weeks after oral administration of 120 ppm DEN to the rat. The VEGF expression appeared at 3 weeks and continuously increased with weeks. The staining of Factor VⅢ R-Ag was negative in any sinusoids in normal control livers, focal or scattered expressed in hyperplastic nodules, and diffusely expressed in hepatocellular carcinomas. As above results, it could be suggested that VEGF expression plays an important role from early period of DEN-induced hepatocarcinogenesis. Also, the expression of VEGF is likely to be associated with phenotypic changes of the sinusoidal endothelium presenting Factor VⅢ expression. However, there was no correlation between the expression pattern of VEGF and the state of metastasis.
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KEYWORD
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Rat hepatocarcinogenesis, N, N-Diethylnitrosamine, Factor Ⅷ, VEGF
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